Sentosa SQ OncoKey Select

Detect sequence variants in pan-cancer samples

INTENDED USE

The Sentosa SQ OncoKey Select Panel (4x16) is a comprehensive Next-Generation Sequencing (NGS)-based assay that simultaneously detects hot spot mutations in formalin-fixed paraffin-embedded (FFPE) samples.

This pan-cancer panel covers 80 genes and more than 500 mutations. Designed by scientists and clinicians the Sentosa OncoKey Select panel covers genes with genes frequently mutated in human cancers and with demonstrated relevance for various ongoing clinical trials. Ultimate goal was a design to serve for sophisticated clinical research, today and in the future.

The Sentosa SQ OncoKey Select panel is intended to be used on the Sentosa SX101 in conjunction with the Sentosa SX FFPE II gDNA Kit and the Sentosa SQ301 instruments. 15 samples can be automatically processed and analysed within 2.5 days (including overnight data analysis). The Sentosa SQ OncoKey Select panel is for research use only (RUO) and not for use in diagnostic procedures.

TARGET GENES

Genes No. of Amplicons Exons
AKT1 1 4
AKT2 1 3
AKT3 3 3, 5, 6
ALK 7 20, 22, 23, 24, 25
APC 10 16
AR 2 5, 8
ARAF 1 7
ARID1A 9 5, 8, 14, 15, 16, 18, 20
BAP1 8 4, 7, 9, 10, 12, 13, 16
BRAF 3 11, 15
BRCA1 2 3, 10
BRCA2 2 11, 27
CDH1 4 2, 3, 6, 14
CDK4 1 2
CDKN2A 3 1, 2
CSF1R 2 7, 22
CTCF 8 3, 4, 5, 6, 7, 8, 10
CTNNB1 1 3
DDR2 1 18
EGFR 8 3, 7, 15, 18, 19, 20, 21
ERBB2 8 8, 17, 18, 19, 20, 21, 22, 24
ERBB3 6 2, 3, 6, 7, 8
ERBB4 5 1, 3, 18, 21, 25
ESR1 4 6, 7, 9, 10
FAT1 2 10, 15
FBXW7 10 4, 5, 7, 8, 9, 10, 11, 12
FGFR1 5 4, 7, 12, 14, 15
FGFR2 6 3, 7, 9, 12, 14
FGFR3 5 7, 9, 14, 16, 18
FOXL2 1 1
GATA3 2 4, 6
GNA11 2 4, 5
GNAQ 2 4, 5
GNAS 4 6, 8, 9, 11
H3F3A 1 1
HIST1H3B 1 1
HNF1A 9 1, 2, 3, 4
HRAS 4 2, 3, 4
IDH1 1 4
IDH2 2 4
JAK2 3 12, 14, 16
KDR 10 7, 8, 11, 15, 22, 23, 24
KEAP1 1 4
KIT 15 2, 8, 9, 10, 11, 12, 13, 14, 17, 18
KMT2C 2 15, 34
KMT2D 4 32, 33, 48, 53
KRAS 3 2, 3, 4
MAP2K1 7 2, 3, 4, 6, 7, 11
MAP2K2 1 2
MAP3K1 3 4, 14, 17
MET 7 13, 14, 16, 19, 20
MLH1 1 12
MTOR 4 24, 39, 47, 53
NF1 4 9, 12, 35, 50
NFE2L2 2 2
NOTCH1 8 6, 8, 26, 27, 34
NRAS 4 2, 3, 4
PDGFRA 4 12, 14, 18
PIK3CA 11 2, 5, 8, 10, 14, 21
PIK3R1 8 9, 10, 11, 12, 13, 14, 15
POLE 2 9, 13
PTEN 23 1, 2, 3, 4, 5, 6, 7, 8, 9
RAC1 3 2, 5, 6
RB1 5 10, 11, 14, 17, 20
RET 7 10, 11, 13, 15, 16
RHOA 3 2, 3
ROS1 2 36, 38
SF3B1 5 14, 15, 16, 18
SMAD4 7 3, 9, 10, 11, 12
SMARCB1 4 2, 4, 5, 9
SMO 4 3, 6, 8, 9
SRC 1 13
STK11 22 1, 2, 3, 4, 5, 6, 7, 8, 9
TP53 23 2, 3, 4, 5, 6, 7, 8, 9, 10, 11
TSC1 1 15
TSC2 2 17, 30
U2AF1 2 2, 6
VHL 3 1, 2, 3

 

FEATURES AND BENEFITS

  • Analyse up to 78 genes in one run
  • Detect 5% variant frequency for mutation
  • Sample input: 5-10 µm section of FFPE sample
  • Get accurate interpretation and reporting with Vela Genomics Service and TheraKey Solution
  • From sample to result in 2.5 days
  • Pay per gene: pay only for your selected genes of interest
  • Combine different solid tumor types in one run
  • Most relevant genes for enhanced tumor testing in one panel
  • Seamless sample traceability and IT connectivity
  • 4 hours of hands on time

WORKFLOW

SPECIFICATIONS (AUTOMATED WORKFLOW)

Attribute Sentosa SQ OncoKey Select Panel
Regulatory Status RUO
Targeted Cancer Pan-Cancer
Number of Genes 78 (incl 3 whole genomes)
Number of Amplicons ~377
Target Mutations ~586
Sample Type FFPE
Sample Input 10 µm section of FFPE sample with > 10% tumor content
Sample Throughput Up to 15 samples + 1 system control
Median Coverage ≥ 500X 
Mutation Detection Sensitivity 5% - 10%
Hands on Time ≤ 4 hours
Turn around Time 2.5 days including overnight sample incubation
Pack Size 4x16

ORDERING INFORMATION

ProductPack SizeItem Number
Sentosa SQ 318 Chip Kit (RUO) 8 runs 300301
Sentosa SQ OncoKey Select Reagents (RUO) 4x16 300692
Sentosa SQ OncoKey Solutions (RUO) 4x16 300693
Sentosa SQ Sequencing Reagents (RUO) 8 300310
Sentosa SQ Sequencing Solutions (RUO) 8 300314
Sentosa SQ Sequencing Supplies (RUO) 8 300304
Sentosa ST Template Beads (RUO) 8 300320
Sentosa ST Template Reagents (RUO) 8 300317
Sentosa ST Template Solutions (RUO) 8 300323
Sentosa ST Template Supplies (RUO) 8 300326
Sentosa SX FFPE II gDNA Kit (RUO) 4x8 tests 300231

For availability in your country, please contact your Vela Diagnostics sales representative. Availability is subject to country's regulatory approval.

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